Composite

Part:BBa_K4695230:Design

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-03)


scfL-his device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal BamHI site found at 960
    Illegal BamHI site found at 2501
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal NgoMIV site found at 1851
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This device uses:

  • AB_T7_lacO promoter (BBa_J435350)
  • BC_BCD23 RBS (BBa_J435385)
  • EF_T7term terminator (BBa_J435361)

All non-coding parts and linkers were chosen from the E. coli Protein Expression Toolkit collection.
This device is compatible with AF vectors.
FLANKED 5' fusion site: GGAG, 3' fusion site: CGCT.

Source

ScfL:
Cellulose binding domain:
Organism: Acetovibrio thermocellus
Structure: https://www.uniprot.org/uniprotkb/Q06851/entry

Cohesin 1:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Cohesin 2:
Organism: Acetivibrio cellulyticus
Structure: https://www.rcsb.org/structure/4UYQ

Cohesin 3:
Organism: Bacteroides cellulosolvens
Structure: https://www.rcsb.org/structure/2Y3N

Registry of standard biological parts


References